Cysteine-390 is the binding site of luminous substance with symplectin, a photoprotein from Okinawan squid, Symplectoteuthis oualaniensis
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Cysteine-390 is the binding site of luminous substance with symplectin, a photoprotein from Okinawan squid, Symplectoteuthis oualaniensis
Symplectin is a photoprotein from a luminous squid, Symplectoteuthis oualaniensis. It has a luminous substrate, dehydrocoelenterazine (DCZ), linked through a thioether bond with a cysteine residue. We have proven the binding site of luminous substrate in symplectin by using an artificial analogue of DCZ, ortho-fluoro-DCZ (F-DCZ). F-DCZ-symplectin emitting strong blue light was reconstituted fro...
متن کاملK/Na-triggered bioluminescence in the oceanic squid Symplectoteuthis oualaniensis.
A distinctive type of luminescent system present in the large dorsal luminous organ of the oceanic squid Symplectoteuthis oualaniensis is described. The organ produces an intense blue flash of light followed by a rapid decay in light intensity. Luminescence originates from numerous oval granules present in the luminous organ. The essential light-emitting components are membrane bound. Intact gr...
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The squid Sthenoteuthis oualaniensis, formerly Symplectoteuthis oualaniensis, generates light using the luciferin coelenterazine and a unique enzyme, symplectin. Genetic information is limited for bioluminescent cephalopod species, so many proteins, including symplectin, occur in public databases only as sequence isolates with few identifiable homologs. As the distribution of the symplectin/pan...
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-1. Our current knowledge of the squid, Symplectoteuthis oualaniensis, one of the organisms utilized to a large extent during the Alpha Helix Kona Expedition, is briefly reviewed. Symplectoteuthis oualaniensis (Lesson, 1830) is a member of the order Teuthoidea, the suborder Oegopsida and the family Ommastrephidae. Squids belonging to this family are among the largest and most numerous of the fa...
متن کاملSite-specific mutagenesis of the calcium-binding photoprotein aequorin.
The luminescent protein aequorin from the jellyfish Aequoria victoria emits light by an intramolecular reaction in the presence of a trace amount of Ca(2+). In order to understand the mechanism of the reaction, a study of structure-function relationships was undertaken with respect to modifying certain of its amino acid residues. This was done by carrying out oligonucleotide-directed site-speci...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 2008
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.84.386